Conference Papers of Professor Dr. Sukumar Bepary
www.amrconference2024.in April 4-5, 2024
National Conference on “Exploring the Bioresources of India to fight against Antimicrobial Resistance (AMR)”
Article No: MRC/2024/A-19
Searching Antimicrobial Activity in 2-Phenoxyacetamide Analogues
MD. Mofazzal Hossain1,2, Bishyajit Kumar Biswas1 and Sukumar Bepary1*
1Department of Pharmacy, Jagannath University, Dhaka, Bangladesh
2Department of Pharmacy, University of Information Technology and Sciences, Dhaka, Bangladesh.
Abstract: Antimicrobial resistance is one of the top global public health issues. It is making infections harder to treat and also resulting in sharp increase in life threatening risks in medical procedures, like, surgery, caesarean sections, chemotherapy, etc. This is also creating serious economic burden worldwide. This study searched the antimicrobial potential of some novel scaffold to meet the ever-increasing global demand of new antibacterial agents to fight against these upcoming infections worldwide. Some novel 2-phenoxyacetamide analogues have been synthesized in laboratory with moderate to high yields (57-96%). These have been subjected to assay of inhibitory potential against Escherichia coli, Salmonella typhi, Staphylococcus aureus and Bacillus megaterium by using disc diffusion method. Then the compounds were tested for the inhibitory potency against two fungi, like, Aspergillus flavus and Aspergillus niger. Subsequently, the compounds have been observed for the in vitro antiinflammatory potency by the denaturation of albumin assay and RBC membrane stabilization assay. Finally the compounds have been tested for analgesic properties by using the acetic acid induced writhing inhibition assay. The phenoxyacetamides gave no zone of inhibition in tests antimicrobial property. But these showed promising antiinflammatory protential in this study. While observing the inhibition of writhing in mice, the compounds showed IC50 values ranging from 1.6 mg/kg to 20.7 mg/kg. In preventing denaturation of egg albumin, they had IC50 values from 103 μg/mL to 294 μg/mL, where diclofenac showed had IC50 as 170 μg/mL. In stabilizing human red blood cells, they had IC50 values of 5.3 μg/mL to 266 μg/mL, The biological study was followed by docking studies for predicting possible interactions in the binding site of Cyclooxygenase enzyme. The phenoxyacetamide derivatives represents a scaffold not having any antimicrobial property. But this represents a promising scaffold that can be further explored for searcing new antiinflammatory pharmacophore for future researches.
Important Links