Teaching Experience

Institution: Department of Genetic  Engineering and Biotechnology, Jagannath University, Dhaka-1100, Bangladesh.

Designation: Lecturer

Duration: 07 March 2021- Till date

 

Research Experience

Organization: Mucosal Immunology and Vaccinology Laboratory, Infectious Diseases Division, International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b), Dhaka-1212, Bangladesh.

Designation: Research Officer

Duration: 4 Year (March 15, 2017 to March 06, 2021)

Project Title: Antigen specific memory B cell responses in Bangladeshi adults after one or two-dose oral killed cholera vaccination and comparison with responses in patients with naturally acquired cholera.

Project Title: A Phase I Double-Blinded, Placebo-Controlled, Dose Escalating study to evaluate the safety and Immunogenicity of Double Mutant Heat-Labile Toxin LTR192G/L211A (dmLT) from ETEC by Vaccination in Adults residing in an endemic area.

Project Title:  Correlates of protection of COVID-19 immunology (COPCI) study.

Molecular Techniques:

● Isolation of Peripheral Blood Mononuclear Cells (PBMCs) and plasma from whole blood by using Ficoll-Isopaque density gradient centrifugation

● ELISPOT assay for antigen specific Antibody Secreting Cells (ASCs)

● Evaluation of Antibody in Lymphocyte Supernatant  (ALS) samples for detection of active cholera infection

● Measurment of antigen specific gut homing ASCs

● Cryopreservation and thawing of the PBMCs

● Memory B cells (MBC) culture

● ELISPOT assay for antigen specific MBC in PBMCs

● Counting of antigen specfic MBC and ASCs by CTL autoanalyzer

● ELISA for LPS Ogawa, Inaba and O139 specific IgA, IgG and IgM antibodies responses in vaccinees

● Vibriocidal antibody assay in plasma

● Immunophetyphing of the MBC and plasmablast cells by Fluorescence-activated Cell Sorting (FACS) assay and analysis of the data via FlowJo software

● Immunophenotyping of memory T cells containing SARS-CoV-2-specific T cells determined by flow cytometry assay

 

Institution: Microbiology, Fermentation and Environmental Biotechnology Laboratory, Department of Genetic  Engineering and Biotechnology, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh.

Designation: Research  Assistant

Duration: 1 Year (January 2016 to February  2017)

Project Title: “Bioconversion of organic municipal solid wastes into bioresources through production of industrially important recombinant enzymes and biodiesel”, funded by TWAS-The World Academy of Sciences, and Ministry of Science and Technology of Bangladesh.

Molecular Techniques:

● Genomic DNA extraction, purification and quantification from blood, bacteria and fungi

● Bacteria and fungi identification based on 16S and 18S rDNA sequence, respectively

● cDNA libraray constructions

● Primer designing

● DNA, RNA and protien electrophoresis

● Plasmid profiling

● Bacteria and fungal culturing

● Restriction digestion and Restriction Fragment Length Polymorphism (RFLP)

● Polymerase Chain Reaction (PCR) for pathogen identifcation and gene isolation

● PCR product purification

● DNA Sequencing

● Enzymes purification (Use and handling of different chromatographic techniques such as, ion exchange using Biologic LP, column chromatography etc.) and characterization

● Extracellular enzyme production in bioreactor, characterization and application in hide processing, raw meat degradation and waste management

● Animal cell culture and plant tissue culture

 

Bioinformatics skills:

● Computational biology using bioinformatics tools and software (Protein modeling and structural analysis, genome-wide analysis for proteins in fungi and bacteria, phylogeny, molecular docking, protein-protein interaction etc)

● Handling and operating different bioinformatics tools such as CLUSTAL Omega, BLAST, Prosite, ExPASy Proteomics Tools, MoE, PYMOL, MEGA, Tree Graph, SOPMA, RCSB PDB, Vaccine Prediction and so on

 

 

Training/Workshop Experience

Biological databases & data mining; Comparing genomes of different species, gene finding; Sequence analysis & functional annotation;  Phylogenetics; Protein structure prediction, modeling and visualization;  Virtual screening for drug discovery;  Molecular docking; Docking output visualization and analysis;  Linux operating system & bash scripting;  Next generation sequencing data analysis;  Basics of R programming & analysis;  Basics of python programming & analysis etc.

 

Training on Manuscript Writing

Duration: January 17, 24 and February 06, 2021

International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b), Mohakhali, Dhaka-1212, Bangladesh.

Trainer: Dr. Jocalyn Clark, MSc, PhD, FRCPE, FCAHS

Executive Editor, The Lancet

Adjunct Professor of Medicine, University of Toronto

Former Executive Editor icddr,b, Bangladesh.

 

Training Course on Good Clinical Practice (GCP)

Duration: November 29, 30, and December 01, 2020

International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b), Mohakhali, Dhaka-1212, Bangladesh.

GCP- Definitions and historical events, ICH- Definitions and glossary,  Principles of ICH-GCP,  Clinical trial- Phase-I, II, III, IV), protocol development, registration and investigator’s brochure,  Ethical principles in clinical research, Roles and responsibilities of different players in clinical trial (Ethics committee, investigator, sponsor, clinical trial monitor), Informed consent and documentation, Clinical safety monitoring and reporting, Confidentiality and data integrity, Supply and handling of the investigational product(s) & export of Biological samples, Audit, regulatory process, DSMB at icddr,b and challenges of CT in Bangladesh etc.

 

Training on Results-Based Management (RBM)

Duration: July 1, 2 and 6, 2020

International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b), Mohakhali, Dhaka-1212, Bangladesh.

Overview of RBM: Context, process, principles, and key elements, Introduction to Logical Framework Approach, Stakeholder analysis and engagement, Problem identification: cause and effect analysis, Formulation of objectives, Intervention logic, indicators, means of verification, Risk analysis and management; assumption; monitoring of results, Performance Measurement and Accountability Framework etc.

 

Basic Course on Structural Biology

Duration: June 26-July 07, 2020

Bangladeshi Structural Biologists.

Course Coordinator: Dr. Munan Sheikh, Associate Director, Biomolecular Science at Cedilla Therapeutics, USA.

Introduction to Protein, Introduction to Structural Biology, Cloning, Protein expression and purification for Structural Biology, Protein Crystallization and X-ray crystallography, X-ray Data collection to get a structure, What is cryo Electron Microscopy (cryoEM), Sample preparation to structure determination, CryoEM data analysis, Small Angle X-ray Scattering (SAXS), Nuclear Magnetic Resonance (NMR) in Structural Biology, Introduction to Electron Paramagnetic Resonance (EPR Spectroscopy), Model building, validation, deposition, What we can learn from a structure, structure analysis.

 

Training on Biosafety and Biosecurity

Duration:  April 19-20, 2017

International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b), Mohakhali, Dhaka-1212, Bangladesh.

Basis of Laboratory Safety, General Laboratory Safety, Chemical Safety, Biosafety and Biosecurity, Waste Management, and Respiratory Protection etc.

 

Training on Basic Biotechnology

Duration:  October 24-29, 2015

National Institute of Biotechnology, Savar, Dhaka, Bangladesh.

Good laboratory practices, Forensic Biotechnology, DNA Sequencing, Biosafety and Intellectual property right, DNA Extraction, Nucleic acid quantification, Restriction digestion, Gel Electrophoresis, PCR, Animal Cell culture, Introductory Bioinformatics and Plant tissue culture techniques.

 

Workshop on “National Integrity Strategy, Service Delivery and Good Governance”

Duration:  May 19, 2016

National Academy for Planning and Development (NAPD) 

Ministry of Planning, Government of the People’s Republic of Bangladesh, 3-A,Nilkhet, Dhaka-1205, Bangladesh.

 

Workshop on “Public Health & You”

Duration: October 10, 2016

School of Public Health-Bloomington, Indiana University, USA.

Introduction to public health, Environmental health, Epidemiology and Biostatistics, Public health administration, Behavioral, Social, and Community Health.